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分离、表征和总 DNA 提取以鉴定真菌异养植物中的内生真菌

JoVE Journal
Biology

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JoVE Journal Biology

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants

Please note that all translations are automatically generated. Click here for the English version.

分离、表征和总 DNA 提取以鉴定真菌异养植物中的内生真菌

DOI:

10.3791/65135-v

•

06:53 min

•

May 05, 2023

•

Laís Soêmis Sisti, Matheus Pena-Passos, Juliana Lishcka Sampaio Mayer

¹LabPlaM – Mycoheterotrophic Plants Research Lab, Department of Plant Biology,State University of Campinas (UNICAMP)

Chapters

00:00Introduction
00:21Isolation of Endophytic Fungi Associated with Plant Organs
03:12Preservation of Purified Fungal Isolates
04:01Micromorphological Characterization (Hyphal Morphology) of Filamentous Fungi
05:10Results: Isolation and Macromorphology Evaluation of Endophytic Fungi Associated with Mycoheterotrophic Plants
06:33Conclusion

Summary

Automatic Translation

本文旨在为植物相关内生真菌的分离、分离株的长期保存、形态学表征和总 DNA 提取提供详细和充分的方案，以便随后进行分子鉴定和宏基因组分析。

Tags

Keywords: Endophytic Fungi Mycoheterotrophic Plants Fungal Isolation Fungal Characterization Total DNA Extraction PDA Culture Medium Antibiotic Supplementation Superficial Disinfection Colony Purification AA Medium Fungal Identification Fungal Subculturing

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