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Isolering, karakterisering og total DNA-ekstraktion for at identificere endofytiske svampe i mycoheterotrofe planter
JoVE Journal
Biology
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JoVE Journal Biology
Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
DOI:

06:53 min

May 05, 2023

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Chapters

  • 00:00Introduction
  • 00:21Isolation of Endophytic Fungi Associated with Plant Organs
  • 03:12Preservation of Purified Fungal Isolates
  • 04:01Micromorphological Characterization (Hyphal Morphology) of Filamentous Fungi
  • 05:10Results: Isolation and Macromorphology Evaluation of Endophytic Fungi Associated with Mycoheterotrophic Plants
  • 06:33Conclusion

Summary

Automatic Translation

Den nuværende artikel har til formål at give detaljerede og passende protokoller til isolering af planteassocierede endofytiske svampe, langsigtet konservering af isolater, morfologisk karakterisering og total DNA-ekstraktion til efterfølgende molekylær identifikation og metagenomiske analyser.

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