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1Center for Vector-borne Infectious Diseases, Department of Microbiology, Immunology, and Pathology,Colorado State University, 2Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases,National Institutes of Health, 3Department of Biological Sciences,Vanderbilt University, 4National Center for Microbiology,Instituto de Salud Carlos III
Chapters
- 00:00Introduction
- 00:16Measuring Fecundity in Female Mosquitoes
- 02:38Measuring Wing Length, Area, and Centroid Size
- 03:59Assessing Egg Fertility
- 04:53Determination of Sex Ratio in the Pupae and Larvae Viability
- 05:32Determination of Larvae-to-Pupae Development Time and Male Contribution
- 06:56Determination of Mosquito Longevity
- 07:25Results: Measurement of Development and Survival Time in Transgenic Aedes aegypti Mosquitoes
- 09:01Conclusion
The present protocol describes how to measure common life parameter data in Aedes aegypti mosquitoes, including fecundity, wing size, fertility, sex ratio, viability, development times, male contribution, and adult longevity. These measurements can be used to assess the fitness of transgenic mosquitoes.
