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En arbejdsgang for lipidnanopartikelformuleringsoptimering (LNP) ved hjælp af designede blandingsproceseksperimenter og selvvaliderede ensemblemodeller (SVEM)
JoVE Journal
Chemistry
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JoVE Journal Chemistry
A Workflow for Lipid Nanoparticle (LNP) Formulation Optimization using Designed Mixture-Process Experiments and Self-Validated Ensemble Models (SVEM)
DOI:

13:54 min

August 18, 2023

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Chapters

  • 00:04Introduction
  • 01:02Recording the Study Purpose, Responses, and Factors
  • 01:31Creation of the Design Table with a Space Filling Design
  • 04:08Running the Experiment
  • 04:22Analyzing the Experimental Results
  • 11:26Confirmation Runs
  • 12:26Representative Results
  • 13:30Conclusion

Summary

Automatic Translation

Denne protokol giver en tilgang til formuleringsoptimering over blandings-, kontinuerlige og kategoriske undersøgelsesfaktorer, der minimerer subjektive valg i den eksperimentelle designkonstruktion. Til analysefasen anvendes en effektiv og brugervenlig modelleringstilpasningsprocedure.

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