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大肠杆菌长期进化实验中的每日转移、存档种群和测量适应性
JoVE Journal
Genetics
Author Produced
This content is Free Access.
JoVE Journal Genetics
Daily Transfers, Archiving Populations, and Measuring Fitness in the Long-Term Evolution Experiment with Escherichia coli
DOI:

15:00 min

August 18, 2023

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Chapters

  • 00:03Introduction
  • 02:02Daily Transfers of LTEE Populations
  • 06:04Competitive Fitness Assays
  • 06:44Prepare Supplies
  • 08:24Day -2: Revive Competitors
  • 09:09Day -1: Precondition Competitors
  • 09:47Day 0: Begin Competition
  • 11:16Alternative:Continue Three-Day Competition
  • 11:43Day 1 or 3: Finish Competition
  • 12:41Calculate and Plot Fitness
  • 12:52Representative Results Appearance and turbidity of LTEE Cultures
  • 13:22Representative Results Co-culture Competition Results
  • 14:39Conclusion

Summary

Automatic Translation

该协议描述了如何通过执行其日常转移和定期冷冻来维持 大肠杆菌 长期进化实验(LTEE),以及如何进行竞争测定以测量进化细菌的适应性改善。这些程序可以作为研究人员开始自己的微生物进化实验的模板。

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