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Journal
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Genetics
/
大肠杆菌
长期进化实验中的每日转移、存档种群和测量适应性
JoVE Journal
Genetics
Author Produced
This content is Free Access.
JoVE Journal
Genetics
Daily Transfers, Archiving Populations, and Measuring Fitness in the Long-Term Evolution Experiment with
Escherichia coli
Please note that all translations are automatically generated.
Click here for the English version.
大肠杆菌
长期进化实验中的每日转移、存档种群和测量适应性
DOI:
10.3791/65342-v
•
15:00 min
•
August 18, 2023
•
Jeffrey E. Barrick
2
,
Zachary D. Blount
3
,
Devin M. Lake
4
,
Jack H. Dwenger
,
Jesus E. Chavarria-Palma
,
Minako Izutsu
3
,
Michael J. Wiser
5
1
Department of Molecular Biosciences
,
The University of Texas at Austin
,
2
BEACON Center for the Study of Evolution in Action
,
Michigan State University
,
3
Department of Microbiology and Molecular Genetics
,
Michigan State University
,
4
Department of Integrative Biology
,
Michigan State University
,
5
Biological Sciences Program
,
Michigan State University
Chapters
00:03
Introduction
02:02
Daily Transfers of LTEE Populations
06:04
Competitive Fitness Assays
06:44
Prepare Supplies
08:24
Day -2: Revive Competitors
09:09
Day -1: Precondition Competitors
09:47
Day 0: Begin Competition
11:16
Alternative:Continue Three-Day Competition
11:43
Day 1 or 3: Finish Competition
12:41
Calculate and Plot Fitness
12:52
Representative Results Appearance and turbidity of LTEE Cultures
13:22
Representative Results Co-culture Competition Results
14:39
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了如何通过执行其日常转移和定期冷冻来维持
大肠杆菌
长期进化实验(LTEE),以及如何进行竞争测定以测量进化细菌的适应性改善。这些程序可以作为研究人员开始自己的微生物进化实验的模板。
Tags
Daily Transfers
Archiving
Fitness Measurement
Long-term Evolution Experiment
Escherichia Coli
Glucose-limited Medium
REL606
REL607
Ara+
Ara-
Colony Color
Co-culture Competition Assays
Relative Fitness
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