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Vertical Immobilization Method for Time-Lapse Microscopy Analysis in Filamentous Cyanobacteria
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Vertical Immobilization Method for Time-Lapse Microscopy Analysis in Filamentous Cyanobacteria

Vertical Immobilization Method for Time-Lapse Microscopy Analysis in Filamentous Cyanobacteria

DOI:
10.3791/65612-v

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07:26 min

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September 25, 2023

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, , ,
1Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas,Pontificia Universidad Católica de Chile, 2Laboratorio de Ciencias Omicas, Facultad de Ciencias de la Salud,Universidad Espíritu Santo

Chapters

  • 00:07Introduction
  • 01:02Protocol
  • 06:32Representative Results
  • 07:02Conclusion

Summary

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We present a simple and accessible method for filamentous cyanobacterial visualization in the XY plane. A low-melting point agarose matrix was used, allowing the acquisition of images of proteins involved in the division, in a vertical orientation. Therefore, this methodology can be applied to any filamentous organism and different kinds of proteins.

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Keywords: Vertical ImmobilizationTime-lapse MicroscopyFilamentous CyanobacteriaAnabaenaCell DivisionZ-ringDivisome ComponentFtsZ-GFPLow-melting-point AgaroseCentrifugationSample Preparation

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