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Medicine
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A Protocol to Isolate, Culture, and Identify Human Limbal Niche Cells
/
Limbal Niş Hücrelerin İzolasyonu ve Tanımlanması
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Medicine
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JoVE Journal
Medicine
Isolation and Identification of Limbal Niche Cells
Please note that all translations are automatically generated.
Click here for the English version.
Limbal Niş Hücrelerin İzolasyonu ve Tanımlanması
A Protocol to Isolate, Culture, and Identify Human Limbal Niche Cells
DOI:
10.3791/65618-v
•
01:28 min
•
October 27, 2023
•
Guanyu Su
,
Wei Wang
,
Lingjuan Xu
,
Rong Liu
,
Yongyao Tan
,
Bihui Jin
,
Chunxiu You
,
Xiao Zhou
,
Yihong Xiong
,
Huatao Xie
,
Guigang Li
1
Department of Ophthalmology, Tongji Hospital, Tongji Medical College
,
Huazhong University of Science and Technology
,
2
Institute of Medicine Nursing
,
Hubei University of Medicine
,
3
Department of Ophthalmology, Union Hospital, Tongji Medical College
,
Huazhong University of Science and Technology
Tags
Limbal Niche Cells
Limbal Stem Cell Deficiency
Single Cell Sequencing
Mesenchymal Stem Cells
Collagenase A
Trypsin-EDTA
Modified Embryonic Stem Cell Medium
Matrigel
Immunofluorescence
Real-time Quantitative PCR
Flow Cytometry
Stem Cell Markers
VIM
CD90
CD105
PDGFR-beta
Pan-CK
CD73
SCF
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