JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Neuroscience
/
High-Resolution 3D Reconstruction of Human Brain with SHORT and LSFM
/
Clarification optique et marquage pour la microscopie à fluorescence à nappe de lumière dans l’imagerie cérébrale humaine à grande échelle
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Neuroscience
Optical Clearing and Labeling for Light-sheet Fluorescence Microscopy in Large-scale Human Brain Imaging
Please note that all translations are automatically generated.
Click here for the English version.
Clarification optique et marquage pour la microscopie à fluorescence à nappe de lumière dans l’imagerie cérébrale humaine à grande échelle
High-Resolution 3D Reconstruction of Human Brain with SHORT and LSFM
DOI:
10.3791/65960-v
•
01:20 min
•
January 26, 2024
•
Danila Di Meo*
1
,
Josephine Ramazzotti*
1
,
Marina Scardigli*
1,2
,
Franco Cheli
,
Luca Pesce
3
,
Niamh Brady
,
Giacomo Mazzamuto
4,5
,
Irene Costantini
4,6
,
Francesco S. Pavone
4,5
1
European Laboratory for Non-linear Spectroscopy (LENS)
,
University of Florence
,
2
Division of Physiology, Department of Experimental and Clinical Medicine
,
University of Florence
,
3
Department of Physics
,
University of Pisa
,
4
National Research Council – National Institute of Optics (CNR-INO)
,
5
Department of Physics and Astronomy
,
University of Florence
,
6
Department of Biology
,
University of Florence
Tags
Keywords: Optical Clearing
Light-sheet Fluorescence Microscopy
Large-scale Human Brain Imaging
3D Cytoarchitecture
Volumetric Reconstruction
Rapid Clearing
Homogeneous Multi-labeling
Neuronal Subpopulations
Micrometer Resolution
High-throughput Protocol
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
check_url/65960?article_type=v
Clarification optique et marquage pour la microscopie à fluorescence à nappe de lumière dans l’imagerie cérébrale humaine à grande échelle
check_url/65960?article_type=v
Préparation de coupes de cerveau humain fixées au formol pour la technique de transformation tissulaire SHORT
check_url/65960?article_type=v
SWITCH - H
2
O
2
- Récupération d’antigène — TDE (SHORT) Technique de transformation tissulaire
check_url/65960?article_type=v
Sample Mounting for Light-Sheet Fluorescence Microscopy and Stripping for Multi-Round Labeling of the Same Specimens
Read Article