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Journal
/
Neuroscience
/
Extraction and Enzymatic Purification of Microglia from the Mouse Spinal Cord
/
Rapid and Efficient Enrichment of Mouse Spinal Cord Microglia
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Rapid and Efficient Enrichment of Mouse Spinal Cord Microglia
Rapid and Efficient Enrichment of Mouse Spinal Cord Microglia
Extraction and Enzymatic Purification of Microglia from the Mouse Spinal Cord
DOI:
10.3791/65961-v
•
01:52 min
•
September 22, 2023
•
Claudia I Gutiérrez-Román
2
,
María E Meléndez Camargo
,
Cuauhtémoc C García Rojas
,
Miguel Jimenez Olvera
,
Sandra H Gutiérrez Román
,
Oscar Medina-Contreras
1
Epidemiology, Endocrinology & Nutrition
,
Mexico Children’s Hospital
,
2
Pharmacy Department, National School of Biological Sciences
,
National Polytechnic Institute
,
3
Graduate Department
,
Dr. Eduardo Liceaga General Hospital of Mexico
,
4
Pain Clinic and Palliative Care
,
Dr. Eduardo Liceaga General Hospital of Mexico
,
5
General Surgery Service
,
Metropolitan Angeles Hospital
Tags
Spinal Cord
Microglia
Purification
Isolation
Flow Cytometry
CD11b
CD45
Collagenase
Density Gradient
Heterogeneity
Central Nervous System
Neurological Pathologies
Chronic Pain
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