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Journal
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Biology
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FACS Analysis of GFP and mCherry Reporter Cells to Evaluate NHEJ & HR Efficiency
/
Author Spotlight: Decoding DNA Repair by Extrachromosomal NHEJ Assay and HR Assays
JoVE Journal
Biology
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JoVE Journal
Biology
Analysis of Nonhomologous End Joining and Homologous Recombination Efficiency in HEK-293T Cells Using GFP-Based Reporter Systems
Author Spotlight: Decoding DNA Repair by Extrachromosomal NHEJ Assay and HR Assays
FACS Analysis of GFP and mCherry Reporter Cells to Evaluate NHEJ & HR Efficiency
DOI:
10.3791/66501-v
•
02:29 min
•
February 02, 2024
•
Lu-Ping Zhang
,
Yong-Hong Nie
,
Tuo Tang
,
Ai-Xue Zheng
,
Xian Hong
,
Tao Wang
1
Laboratory of Protein Structure and Function, Institute of Medicine and Pharmacy
,
Qiqihar Medical University
Tags
NHEJ
Homologous Recombination
DNA Double-strand Breaks
GFP Reporter System
HEK-293T Cells
Flow Cytometry
Extrachromosomal Assay
I-SceI Endonuclease
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