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Journal
/
Bioengineering
/
Purification of AAV Vectors by Single-Step Heparin Affinity Chromatography
/
Adeno ile İlişkili Viral Vektörlerin Tek Adımlı ve Yarı Otomatik Heparin Afinite Kromatografisi Protokolü ile İzolasyonu
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol
Please note that all translations are automatically generated.
Click here for the English version.
Adeno ile İlişkili Viral Vektörlerin Tek Adımlı ve Yarı Otomatik Heparin Afinite Kromatografisi Protokolü ile İzolasyonu
Purification of AAV Vectors by Single-Step Heparin Affinity Chromatography
DOI:
10.3791/66550-v
•
01:57 min
•
April 05, 2024
•
Miguel M. Lopes*
1,2,3
,
Sara M. Lopes*
1,2,3
,
Rafael Baganha
2,4
,
Carina Henriques
2,5
,
Ana C. Silva
2,3
,
Diana D. Lobo
2,3
,
Luísa Cortes
2,3,6
,
Luís Pereira de Almeida*
1,2,4,5
,
Rui Jorge Nobre*
1,2,3,4
1
CNC-UC – Center for Neuroscience and Cell Biology
,
University of Coimbra
,
2
CIBB – Center for Innovative Biomedicine and Biotechnology
,
University of Coimbra
,
3
IIIUC – Institute for Interdisciplinary Research
,
University of Coimbra
,
4
ViraVector – Viral Vectors for Gene Transfer Core Facility
,
University of Coimbra
,
5
FFUC – Faculty of Pharmacy
,
University of Coimbra
,
6
MICC-CNC – Microscopy Imaging Center of Coimbra – CNC
,
University of Coimbra
Tags
Adeno-associated Viral Vectors
AAV Purification
Heparin Affinity Chromatography
Transient Transfection
Density Gradient Ultracentrifugation
Scalable Production
Semi-automated Protocol
Mosaic AAV
Preclinical Studies
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