JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
本地化的RNAi和药用水蛭异位基因表达
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Localized RNAi and Ectopic Gene Expression in the Medicinal Leech
Please note that all translations are automatically generated.
Click here for the English version.
本地化的RNAi和药用水蛭异位基因表达
DOI:
10.3791/697-v
•
16:19 min
•
April 17, 2008
•
Orit Shefi
,
Claire Simonnet
,
Alex Groisman
,
Eduardo R Macagno
1
Division of Biological Sciences
,
University of California San Diego – UCSD
,
2
Department of Physics
,
University of California San Diego – UCSD
Chapters
00:00
Biolistic Gene Delivery and the Medicinal Leech
01:27
Introduction
03:04
The medicinal leech
08:33
Preparing the Experimental Set Up
10:19
Loading the gun with the pre-coated particles
11:17
Animal preparation and delivery of dsRNA-coated particles
14:25
Assessing knockdown gene delivery
15:13
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这个视频中,我们展示一个准确的枪法交付到现场组织了一种新型微型基因枪试剂的过程。我们撞倒在水蛭胚胎提供腹侧体壁和神经节单节段双链RNA分子的轴突导向分子Netrin的表达。
Tags
Localized RNAi
Ectopic Gene Expression
Medicinal Leech
Pneumatic Capillary Gun
Biolistic Delivery
Gene Expression Patterns
Leech Embryos
Internal Controls
Substances Introduction
Lateral Dimensions
Penetration Depth
Knocking Down
Knocking In
Axon Guidance Molecule Netrin
DsRNA
Plasmid-DNA
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
check_url/697?article_type=v
主电穿孔的小鼠骨髓造血细胞培养制备
check_url/697?article_type=v
脊髓电
check_url/697?article_type=v
使用基因脉冲MXcell电穿孔系统主细胞的转染效率高
check_url/697?article_type=v
机器人技术和植物组织的基因表达研究的动态图像分析
check_url/697?article_type=v
使用自动细胞计数器简化基因表达研究:在Namalwa细胞的IL - 4依赖的基因表达的siRNA击倒
check_url/697?article_type=v
石蜡包埋及冷冻的部分
果蝇
成人肌肉
check_url/697?article_type=v
在体外
重组活跃
T。谷盗
端粒酶
check_url/697?article_type=v
通过显微注射介导的RNAi Necromenic线虫基因敲除和转基因
Pristionchus pacificus</em
check_url/697?article_type=v
全球合成的RNA在单细胞水平以下的低氧分析
check_url/697?article_type=v
代谢率的测量<em>果蝇</em>使用呼吸运动
Read Article