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本地化的RNAi和药用水蛭异位基因表达
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JoVE Journal Biology
Localized RNAi and Ectopic Gene Expression in the Medicinal Leech
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本地化的RNAi和药用水蛭异位基因表达

DOI:
10.3791/697-v

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16:19 min

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April 17, 2008

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, , ,
1Division of Biological Sciences,University of California San Diego – UCSD, 2Department of Physics,University of California San Diego – UCSD

Chapters

  • 00:00Biolistic Gene Delivery and the Medicinal Leech
  • 01:27Introduction
  • 03:04The medicinal leech
  • 08:33Preparing the Experimental Set Up
  • 10:19Loading the gun with the pre-coated particles
  • 11:17Animal preparation and delivery of dsRNA-coated particles
  • 14:25Assessing knockdown gene delivery
  • 15:13Conclusion

Summary

Automatic Translation

Automatic Translation

在这个视频中，我们展示一个准确的枪法交付到现场组织了一种新型微型基因枪试剂的过程。我们撞倒在水蛭胚胎提供腹侧体壁和神经节单节段双链RNA分子的轴突导向分子Netrin的表达。

Tags

Localized RNAiEctopic Gene ExpressionMedicinal LeechPneumatic Capillary GunBiolistic DeliveryGene Expression PatternsLeech EmbryosInternal ControlsSubstances IntroductionLateral DimensionsPenetration DepthKnocking DownKnocking InAxon Guidance Molecule NetrinDsRNAPlasmid-DNA

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