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Principles of Site-Specific Recombinase (SSR) Technology
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Principles of Site-Specific Recombinase (SSR) Technology

Principles of Site-Specific Recombinase (SSR) Technology

DOI:
10.3791/718-v

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07:06 min

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May 29, 2008

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1Max Plank Institute for Molecular Cell Biology and Genetics, Dresden

Chapters

  • 00:04Introduction
  • 00:33Personal Background / Training
  • 01:02Max Planck Institute of Molecular Cell Biology and Genetics, Dresden
  • 02:00Site-specific recombinase technology: origins
  • 03:23Site-specific recombinase technology: conditional gene knockout
  • 06:24Site-specific recombinase technology towars therapy

Summary

Automatic Translation

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The advent of site-specific recombinase (SSR) technology and the Cre/lox system has led to numerous advances in molecular biology, and has proven itself as a valuable tool for assessing gene function in transgenic animals. This interview discusses the mechanism of site specific recombination by Cyclization recombinase (Cre) and how the use of this enzyme has led to the development of conditional mutagenesis, which has significant advantages over traditional knock out strategies.

Tags

Site-specific Recombinase (SSR) TechnologyGene Structure ManipulationGene Function ExplorationMolecular Biology ToolCre/lox SystemCre RecombinaseLoxP SitesRecombination EventDNA RearrangementGene Deletion

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