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Electrophoretic Separation of Proteins
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Electrophoretic Separation of Proteins
June 12th, 2008
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Chapters
Summary
In this video, we demonstrate a method for electrophoretic separation of proteins using poly-acrylimide gel electrophoresis (PAGE).
Tags
Electrophoretic Separation Proteins Protein Separation Electrophoresis Complex Mixtures Subunit Compositions Protein Samples Purification Of Proteins Polyacrylamide Gel Electrophoresis Electrical Field Pore Size Acrylamide Concentration Protein Charge Protein Size Protein Shape Migration Rate Laemmli Method Discontinuous Gel Electrophoresis Denaturing Conditions Sodium Dodecyl Sulfate (SDS)Related Videos
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Research • BiologygDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair
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Research • GeneticsA Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
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Research • Cancer ResearchNext Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
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Research • BiologyIn vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
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Research • BiologyAnalysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection
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Research • BiologyMeasuring the Kinetics of mRNA Transcription in Single Living Cells
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Research • BiologyGenome-wide Analysis of Aminoacylation (Charging) Levels of tRNA Using Microarrays
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Research • Immunology and InfectionIn vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme
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Research • BiochemistryAn In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity
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Research • BiochemistryProtein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function
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Research • BiologyRNA Isolation from Embryonic Zebrafish and cDNA Synthesis for Gene Expression Analysis
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Research • BiologyAnalysis of Translation Initiation During Stress Conditions by Polysome Profiling
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Research • BiologyAssessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling
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Research • GeneticsTools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks
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Research • BiologyTelomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
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Research • BiologyMethods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1