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从人类胚胎干细胞的染色质免疫沉淀
JoVE Journal
Biology
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JoVE Journal Biology
Chromatin Immunoprecipitation from Human Embryonic Stem Cells
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从人类胚胎干细胞的染色质免疫沉淀

DOI:
10.3791/780-v

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10:36 min

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July 22, 2008

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, ,
1Department of Biochemistry,University of California – Riverside

Chapters

  • 00:01Title
  • 00:11Introduction
  • 00:27Formaldehyde Crosslinking Cells
  • 02:22Preparing Magnetic Beads
  • 04:13Cell Sonication
  • 05:58Chromatin Immunoprecipitation, Wash, and Elution
  • 08:53Crosslink Reversal and DNA Purification
  • 09:58Conclusion

Summary

Automatic Translation

Automatic Translation

恰逢的ESC分化与细胞类型的具体结构的变化和染色质组成。定义stemcellness和细胞分化的机制，这些变化的检测提供了有价值的见解。染色质免疫沉淀（ChIP）代表一个有价值的方法来剖析底层干细胞分化的分子机制。

Tags

Chromatin ImmunoprecipitationHuman Embryonic Stem CellsStem CellsSelf-renewalMultipotencyDifferentiationTranscription FactorsSignaling MoleculesGene Expression PatternsCell Differentiation ProcessChromatin StructureHistonesEpigenetic FactorsHistone VariantsTranscription FactorsChromatin Immunoprecipitation (ChIP)

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