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Chapters
- 00:00Title
- 00:14Introduction
- 01:00Preparing Tools and Reagents
- 05:03Dissecting Midbrain of P0-P2 Rodent
- 09:10Triturating Brain Segments
- 10:49Plating Dissociated Neurons
- 12:53Primary Dissociated Midbrain Dopamine Cultures
- 13:28Conclusion
Primaria dissociato mesencefalo colture di cellule della dopamina permettono per lo studio delle caratteristiche presinaptici dei neuroni dopaminergici. Possono essere utilizzati per monitorare in tempo reale la cinetica di rilascio di dopamina e proteine / mRNA livelli di regolatori di esocitosi dopamina. Qui vi mostriamo come generare queste culture da neonati di roditori.
Tags
Primary Cell CulturesDopamine NeuronsPresynaptic CharacteristicsDopamine Release KineticsCarbon Fiber AmperometryDopamine Related GenesProteinsQuantitative PCRImmunocytochemistryRodent NeonatesCortical Glial AstrocytesConditioning Of Neuronal Cell Culture MediaMidbrain DissectionDigestionExtractionPlating Of Dopamine NeuronsNeurotrophic FactorsElectrophysiologyVideo MicroscopyCell Viability AssaysToxicological Screens
