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Journal
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Biology
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人类胚胎干细胞：启动文化冷冻细胞
JoVE Journal
Biology
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JoVE Journal
Biology
Human ES cells: Starting Culture from Frozen Cells
Please note that all translations are automatically generated.
Click here for the English version.
人类胚胎干细胞：启动文化冷冻细胞
DOI:
10.3791/86-v
•
02:47 min
•
November 09, 2006
•
Erin Trish
,
John Dimos
,
Kevin Eggan
1
Department of Molecular and Cell Biology
,
Harvard
Chapters
00:03
Prepare media
00:17
Take cells from freezer and thaw
01:58
Start culture
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里，我们证明，我们的实验室如何从冻结的股票开始一个色调的人类胚胎干细胞线文化。
Tags
Human ES Cells
Culture
Frozen Cells
Embryonic Stem Cell Line
HuES Media
Mouse Embryonic Fibroblast Feeder Cells
Serum
Cell Debris
Liquid Nitrogen Storage
-80C Freezer
Thawing
Freezing Media
DMSO
Differentiation
Suspension
Seeding
MEF Plate
Incubator
Media Replacement
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