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Chapters
- 00:00Title
- 00:49Introduction
- 01:16Fixing and Dehydrating the Embryos
- 03:28Prehybridzation
- 05:15Hybridizing Embryos with DIG- and FITC- Coupled Probes
- 06:41DIG Antibody Incubation and Color Reaction
- 09:40FITC Antibody Incubation and Color Reaction
- 11:52Fixation and Processing
- 13:34Results
- 15:08Conclusion
Deze video toont twee-kleuren ganse berg in situ hybridisatie, een methode waarmee de ruimtelijke en temporele expressie patroon van twee verschillende genen kunnen worden gevisualiseerd in jonge kippenembryo's. Deze methode werd oorspronkelijk geïntroduceerd door David Wilkinson, Domingos Henrique, Phil Ingham en David Ish-Horowicz.
Tags
Double Whole Mount In Situ HybridizationEarly Chick EmbryosEmbryonic DevelopmentGene ExpressionBrain DevelopmentNeural Tube FormationSomite FormationHeart Primordia Formation2-color Whole Mount In Situ HybridizationEmbryo DissectionParaformaldehyde FixationPrehybridizationProbe HybridizationDIG ProbeFITC ProbeOvernight HybridizationDIG Coupled AntibodyFITC Coupled AntibodyColor Reaction For DIG SubstrateColor Reaction With FITCPhotograph And SectioningTroubleshooting Guide
