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从美国的马蹄蟹,鲎波吕斐摩斯采血车
JoVE Journal
Biology
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JoVE Journal Biology
Blood Collection from the American Horseshoe Crab, Limulus Polyphemus
DOI:

12:48 min

October 13, 2008

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Chapters

  • 00:00Introduction
  • 01:34Maintaining Horseshoe crabs for research
  • 02:22Basic Limulus Anatomy
  • 03:58Prepare Horseshoe crab for bleeding
  • 05:25Bleeding a Horseshoe crab
  • 07:49Plasma Preparation and Storage
  • 09:46Making explant cultures of aggregated ameobocytes
  • 11:16Conclusion

Summary

Automatic Translation

美国鲎,鲎波吕斐摩斯,无疑是最方便的任何无脊椎动物的血液大量源。血液成分简单,只有一个循环,颗粒amebocyte,只有三个蛋白质丰富的血浆,血蓝蛋白,C -反应蛋白和α2-巨球蛋白的细胞类型。发自内心的血液收集和离心分离血细胞和血浆。

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