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Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
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Bioengineering
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JoVE Journal Bioengineering
Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

DOI:
10.3791/50575-v

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06:21 min

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July 12, 2013

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, ,
1School of Chemistry and Biochemistry,Georgia Institute of Technology

Chapters

  • 00:05Title
  • 01:20DESI Optimization
  • 03:09Tissue Imaging and Image Processing
  • 04:37Results: Imaging of Rat Brain Tissue by DESI-MS
  • 05:56Conclusion

Summary

Automatic Translation

Automatic Translation

Desorption electrospray ionization mass spectrometry (DESI-MS) is an ambient method by which samples, including biological tissues, can be imaged with minimal sample preparation. By rastering the sample below the ionization probe, this spray-based technique provides sufficient spatial resolution to discern molecular features of interest within tissue sections.

Tags

Mass Spectrometry ImagingDESIDesorption Electrospray IonizationAmbient IonizationRat BrainSample PreparationSpatial ResolutionMolecular Information

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