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Methods to Examine the Lymph Gland and Hemocytes in Drosophila Larvae
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Chapters
- 00:05Title
- 00:44Hemolymph Collection to Determine Concentration
- 01:45Hemolymph Collection and Immunochemistry
- 04:32Larval Lymph Gland Dissection
- 06:31Antibody Staining of Larval Lymph Glands
- 08:32Mounting Lymph Gland Tissue for Microscopy
- 09:32Results: Quantification and Imaging of Hemocytes, and Imaging of Dissected and Immunostained Lymph Gland Tissue
- 11:19Conclusion
Drosophila and mammalian hematopoietic systems share many common features, making Drosophila an attractive genetic model to study hematopoiesis. Here we demonstrate dissection and mounting of the major larval hematopoietic organ for immunohistochemistry. We also describe methods to assay various larval hematopoietic compartments including circulating hemocytes and sessile crystal cells.
