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3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration
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Chapters
- 00:05Title
- 01:05Magnetic Device Construction and Stem Cell Labeling
- 02:45Magnetic Cell Seeding
- 04:59Chondrocytes Differentiation
- 07:24Results: Representative Cartilage Regeneration Analyses
- 08:28Conclusion
Chondrogenesis from stem cells requires fine tuning the culture conditions. Here, we present a magnetic approach for condensing cells, an essential step to initiate chondrogenesis. In addition, we show that dynamic maturation in a bioreactor applies mechanical stimulation to the cellular constructs and enhances cartilaginous extracellular matrix production.
Tags
Keywords:
3D Magnetic Stem Cell AggregationCartilage RegenerationMesenchymal Stromal CellsCell CondensationCellular BioreactorDynamic MaturationMuscle RepairCardiac RepairVascular RepairMicromagnet DeviceScaffold SeedingIron Oxide NanoparticlesMagnetic Cell LabelingCell AggregationCell CultureTrypsin-EDTACell PelletCell CountingGlass Bottom Petri Dish
