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Journal / Developmental Biology / Quantifying Branching Density in Rat Mammary…
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Developmental Biology

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JoVE Journal Developmental Biology
Quantifying Branching Density in Rat Mammary Gland Whole-mounts Using the Sholl Analysis Method
 

Quantifying Branching Density in Rat Mammary Gland Whole-mounts Using the Sholl Analysis Method

Article DOI: 10.3791/55789-v • 11:02 min • July 12th, 2017
July 12th, 2017 •
1, 1
1National Toxicology Program Laboratory, Division of the National Toxicology Program, National Institute of Environmental Health Sciences

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Summary
Automatic Translation

Mammary gland development in the rodent has typically been evaluated using descriptive assessments or by measuring basic physical attributes. Branching density is an indicator of mammary development that is difficult to quantify objectively. This protocol describes a reliable method for the quantitative assessment of mammary gland branching characteristics.

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Keywords: Mammary Gland Branching Density Branching Decay Sholl Analysis Computer-assisted Method Whole-mount Images ImageJ Glandular Epithelium Lymph Node Color Channels Background Subtraction Unsharp Mask Noise Removal
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