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Micropuncture of Bowman’s Space in Mice Facilitated by 2 Photon Microscopy
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JoVE Journal Biology
Micropuncture of Bowman’s Space in Mice Facilitated by 2 Photon Microscopy

Micropuncture of Bowman’s Space in Mice Facilitated by 2 Photon Microscopy

DOI:
10.3791/58206-v

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07:37 min

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October 11, 2018

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, , , , , ,
1Anesthesiology & Perioperative Medicine,Oregon Health & Science University, 2Environmental and Biological Services Division,Pacific Northwest National Laboratory, 3Operative Care Division,Portland Veterans Affairs Medical Center

Chapters

  • 00:04Title
  • 00:42Kidney Stabilization and Injection
  • 02:35Glomerulus Selection and Bowman s Space Fluid Aspiration
  • 05:41Results: Representative Bowman s Space Micropuncture and Analysis
  • 07:11Conclusion

Summary

Automatic Translation

Automatic Translation

We present use of 2-photon microscopy to place a micropipette within Bowman's urinary space in mice, combining 2 foundational techniques of renal physiology. Use of 2-photon microscopy overcomes critical limitations of conventional microscopy for micropuncture renal physiology studies.

Tags

Keywords: MicropunctureBowman’s SpaceTwo-photon MicroscopySingle-nephron PhysiologyGlomerular FiltrationTubular PhysiologyMouse PreparationKidney StabilizationFITC-DextranTarget GlomerulusPipette Positioning

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