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Real-time Visualization and Analysis of Chondrocyte Injury Due to Mechanical Loading in Fully Intact Murine Cartilage Explants
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Bioengineering
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JoVE Journal Bioengineering
Real-time Visualization and Analysis of Chondrocyte Injury Due to Mechanical Loading in Fully Intact Murine Cartilage Explants

Real-time Visualization and Analysis of Chondrocyte Injury Due to Mechanical Loading in Fully Intact Murine Cartilage Explants

DOI:
10.3791/58487-v

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08:42 min

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January 07, 2019

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, ,
1Department of Biomedical Engineering,University of Rochester

Chapters

  • 00:04Title
  • 00:44Distal Femur Collection
  • 03:08Proximal Humerus Collection
  • 04:45Live (Calcein AM)/Dead (Propidium Iodide) Staining and Mechanical Testing
  • 07:29Results: Representative Femoral and Humeral Articular Surface Injury Analyses
  • 08:03Conclusion

Summary

Automatic Translation

Automatic Translation

We present a method to assess the spatial extent of cell injury/death on the articular surface of intact murine joints after application of controlled mechanical loads or impacts. This method can be used to investigate how osteoarthritis, genetic factors and/or different loading regimens affect the vulnerability of in situ chondrocytes.

Tags

Real-time VisualizationChondrocyte InjuryMechanical LoadingMurine Cartilage ExplantsArticular ChondrocytesMouse ModelFemur DissectionKnee JointCartilage DegenerationMechanical Injury

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