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Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood
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Immunology and Infection
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JoVE Journal Immunology and Infection
Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

DOI:
10.3791/58689-v

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09:40 min

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November 28, 2018

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, ,
1Virus and Prion Research Unit, National Animal Disease Center,USDA, Agricultural Research Service, 2ORAU/ORISE

Chapters

  • 00:04Title
  • 00:48Processing of Swine Blood Samples and Organic Extraction for Total RNA and Small RNA
  • 02:05Total RNA Isolation Procedure
  • 03:33Globin Reduction
  • 07:24Results: Expression Libraries of the Globin-depleted and Ribo-depleted Whole Blood Samples
  • 08:50Conclusion

Summary

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Here, we present a protocol optimized for the processing of coding (mRNA) and non-coding (ncRNA) globin reduced RNA-seq libraries from a single whole blood sample.

Tags

RNA ClassesCoding RNANon-coding RNAWhole BloodHost-pathogen InteractionsRNAseqGlobin-reducedMRNAMiRNAIsolationCentrifugationLysisPhenol-chloroformEthanolFilter CartridgeWash Solution

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