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Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody
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Cancer Research
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JoVE Journal Cancer Research
Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody

Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody

DOI:
10.3791/60727-v

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07:36 min

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May 16, 2020

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, , , ,
1Laboratory of Novel Biologics,University of Virginia School of Medicine, 2Department of Biochemistry and Molecular Genetics, UVA Cancer Center,University of Virginia School of Medicine, 3Center for Comparative Medicine,University of Virginia

Chapters

  • 00:04Introduction
  • 01:02Expression and Purification of Antibodies
  • 03:22Fluorescent Labeling
  • 04:10Antibody Localization Using an In Vivo Imaging System
  • 06:05Results: Live Imaging of Human OVCAR3 Tumor Bearing Mice
  • 06:56Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol to study the in vivo localization of antibodies in mice tumor xenograft models.

Tags

Antibody DistributionTumor LocalizationAntibody ScreeningCHO Cell ExpressionAntibody PurificationProtein A ColumnReal-time MonitoringSPECTPETCost-effective Technique

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