Journal
/
Cancer Research
/
Discovery of Metastatic Regulators using a Rapid and Quantitative Intravital Chick Chorioallantoic Membrane Model
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Discovery of Metastatic Regulators using a Rapid and Quantitative Intravital Chick Chorioallantoic Membrane Model

Discovery of Metastatic Regulators using a Rapid and Quantitative Intravital Chick Chorioallantoic Membrane Model

DOI:
10.3791/62077-v

•

07:03 min

•

February 03, 2021

•

, , ,
1Department of Oncology,University of Alberta, Edmonton, Alberta, Canada, 2Department of Cell Biology & Anatomy, Cumming School of Medicine,University of Calgary

Chapters

  • 00:05Introduction
  • 00:41Intravenous Injection of Cancer Cells for Metastatic Colony Formation
  • 03:06Embryo Maintenance During the Metastatic Colony Growth and their Isolation
  • 05:26Results: Metastatic Colony Isolation for Different Steps of the Screening Protocol
  • 06:34Conclusion

Summary

Automatic Translation

Automatic Translation

This is an effective method to screen for suppressors or drivers of cancer metastasis. Cells, transduced with an expression library, are injected into the chicken chorioallantoic membrane vasculature to form metastatic colonies. Colonies having decreased or increased invasiveness are excised, expanded, reinjected to confirm their phenotype, and finally, analyzed using high throughput sequencing.

Tags

Keywords: IntravitalChick Chorioallantoic MembraneMetastatic RegulatorsCancer InvasionTherapeutic Gene TargetsCancer MetastasisIV InjectionBorosilicate NeedleCAM SurfaceCancer Cell Suspension

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62077?article_type=v

Advanced Animal Model of Colorectal Metastasis in Liver: Imaging Techniques and Properties of Metastatic Clones
check_url/62077?article_type=v

A Syngeneic Mouse Model of Metastatic Renal Cell Carcinoma for Quantitative and Longitudinal Assessment of Preclinical Therapies
check_url/62077?article_type=v

Murine Model of Metastatic Liver Tumors in the Setting of Ischemia Reperfusion Injury
check_url/62077?article_type=v

Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane
check_url/62077?article_type=v

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
check_url/62077?article_type=v

Using the Chicken Chorioallantoic Membrane In Vivo Model to Study Gynecological and Urological Cancers
check_url/62077?article_type=v

A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery
check_url/62077?article_type=v

Quantifying the Brain Metastatic Tumor Micro-Environment using an Organ-On-A Chip 3D Model, Machine Learning, and Confocal Tomography
check_url/62077?article_type=v

An Ex Vivo Brain Slice Model to Study and Target Breast Cancer Brain Metastatic Tumor Growth
check_url/62077?article_type=v

Monitoring Breast Cancer Growth and Metastatic Colony Formation in Mice using Bioluminescence

Read Article