Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells

October 27th, 2023

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Plating CFSE-Labelled Tumor Cells and Co-Culturing CAR-Expressing Jurkats for In Vitro Cytotoxicity Evaluation (Video) | JoVE

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Preparation of Plate for Fluorescent Imaging and Cell Viability Assessment in CAR-Jurkat Co-Culture with CFSE-Labeled Tumor Cells (Video) | JoVE

04:00 min

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Siddharth Subham*^1,2,3, John D. Jeppson*¹, Benjamin K. Gibbs⁴, Jacqueline Babai², Riza Alker¹, Andrew K. Godwin^4,5,6, David Akhavan^1,2,3

¹Department of Radiation Oncology, University of Kansas Cancer Center, ²Department of Cancer Biology, University of Kansas Cancer Center, ³BioEngineering Program, University of Kansas, ⁴Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, ⁵University of Kansas Cancer Center, ⁶Kansas Institute for Precision Medicine, University of Kansas Medical Center

* These authors contributed equally

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단일 종양 항원을 표적으로 하는 키메라 항원 수용체(CAR)를 발현하는 Jurkat 세포에 의한 정량적 종양 세포 사멸을 평가하기 위한 프로토콜입니다. 이 프로토콜은 말초 혈액 유래 T 세포에서 확인하기 전에 CAR hinge 구조를 신속하게 최적화하기 위한 스크리닝 플랫폼으로 사용할 수 있습니다.

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Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells

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