W. Andy Tao Department of Biochemistry Purdue University Biography Publications Institution JoVE Articles W. Andy Tao has not added a biography. If you are W. Andy Tao and would like to personalize this page please email our Author Liaison for assistance. Publications Plasma-Derived Extracellular Vesicle Phosphoproteomics Through Chemical Affinity Purification Journal of Proteome Research. May, 2020 | Pubmed ID: 32396726 A Phosphorylation-based Switch Controls TAA1-mediated Auxin Biosynthesis in Plants Nature Communications. 02, 2020 | Pubmed ID: 32015349 A RAF-SnRK2 Kinase Cascade Mediates Early Osmotic Stress Signaling in Higher Plants Nature Communications. 01, 2020 | Pubmed ID: 32001690 Mapping Proteome-wide Targets of Protein Kinases in Plant Stress Responses Proceedings of the National Academy of Sciences of the United States of America. Feb, 2020 | Pubmed ID: 31992638 Sequential Phosphoproteomics and N-glycoproteomics of Plasma-derived Extracellular Vesicles Nature Protocols. 01, 2020 | Pubmed ID: 31863077 Tracking Pathogen Infections by Time-Resolved Chemical Proteomics Angewandte Chemie (International Ed. in English). 02, 2020 | Pubmed ID: 31769915 Methyltransferase-like 21c Methylates and Stabilizes the Heat Shock Protein Hspa8 in Type I Myofibers in Mice The Journal of Biological Chemistry. 09, 2019 | Pubmed ID: 31346037 Methyltransferase-like 21e Inhibits 26S Proteasome Activity to Facilitate Hypertrophy of Type IIb Myofibers FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology. 08, 2019 | Pubmed ID: 31162944 Arabinose Biosynthesis is Critical for Salt Stress Tolerance in Arabidopsis The New Phytologist. 10, 2019 | Pubmed ID: 31009077 Leucine-rich Repeat Extensin Proteins Regulate Plant Salt Tolerance in Proceedings of the National Academy of Sciences of the United States of America. 12, 2018 | Pubmed ID: 30514814 Arabidopsis AGDP1 Links H3K9me2 to DNA Methylation in Heterochromatin Nature Communications. 10, 2018 | Pubmed ID: 30382101 High-Throughput Phosphorylation Screening and Validation Through Ti(IV)-Nanopolymer Functionalized Reverse Phase Phosphoprotein Array Analytical Chemistry. 09, 2018 | Pubmed ID: 30103608 Highly Efficient Phosphoproteome Capture and Analysis from Urinary Extracellular Vesicles Journal of Proteome Research. 09, 2018 | Pubmed ID: 30080416 Universal Plant Phosphoproteomics Workflow and Its Application to Tomato Signaling in Response to Cold Stress Molecular & Cellular Proteomics : MCP. 10, 2018 | Pubmed ID: 30006488 Arabidopsis Duodecuple Mutant of PYL ABA Receptors Reveals PYL Repression of ABA-Independent SnRK2 Activity Cell Reports. 06, 2018 | Pubmed ID: 29898403 Analytical Pipeline for Discovery and Verification of Glycoproteins from Plasma-Derived Extracellular Vesicles As Breast Cancer Biomarkers Analytical Chemistry. 05, 2018 | Pubmed ID: 29629753 Reciprocal Regulation of the TOR Kinase and ABA Receptor Balances Plant Growth and Stress Response Molecular Cell. 01, 2018 | Pubmed ID: 29290610 Acquisition of Cholangiocarcinoma Traits During Advanced Hepatocellular Carcinoma Development in Mice The American Journal of Pathology. 03, 2018 | Pubmed ID: 29248454 Structure of the Arabidopsis JMJ14-H3K4me3 Complex Provides Insight into the Substrate Specificity of KDM5 Subfamily Histone Demethylases The Plant Cell. 01, 2018 | Pubmed ID: 29233856 Identification of Upstream Kinases by Fluorescence Complementation Mass Spectrometry ACS Central Science. Oct, 2017 | Pubmed ID: 29104924 Recent Advances in Phosphoproteomics and Application to Neurological Diseases The Analyst. Nov, 2017 | Pubmed ID: 29094114 MAP Kinase Cascades Regulate the Cold Response by Modulating ICE1 Protein Stability Developmental Cell. 12, 2017 | Pubmed ID: 29056551 EZH2 Modifies Sunitinib Resistance in Renal Cell Carcinoma by Kinome Reprogramming Cancer Research. 12, 2017 | Pubmed ID: 28978636 A Protein Complex Regulates RNA Processing of Intronic Heterochromatin-containing Genes in Proceedings of the National Academy of Sciences of the United States of America. 08, 2017 | Pubmed ID: 28808009 Identification of Plant Kinase Substrates Based on Kinase Assay-Linked Phosphoproteomics Methods in Molecular Biology (Clifton, N.J.). 2017 | Pubmed ID: 28730489 The SnRK2 Kinases Modulate MiRNA Accumulation in Arabidopsis PLoS Genetics. 04, 2017 | Pubmed ID: 28419088 Estimating the Efficiency of Phosphopeptide Identification by Tandem Mass Spectrometry Journal of the American Society for Mass Spectrometry. 06, 2017 | Pubmed ID: 28283928 Phosphoproteins in Extracellular Vesicles As Candidate Markers for Breast Cancer Proceedings of the National Academy of Sciences of the United States of America. 03, 2017 | Pubmed ID: 28270605 MST50 is Involved in Multiple MAP Kinase Signaling Pathways in Magnaporthe Oryzae Environmental Microbiology. 05, 2017 | Pubmed ID: 28244240 Kinase Assay-Linked Phosphoproteomics: Discovery of Direct Kinase Substrates Methods in Enzymology. 2017 | Pubmed ID: 28137576 A Pair of Transposon-derived Proteins Function in a Histone Acetyltransferase Complex for Active DNA Demethylation Cell Research. Feb, 2017 | Pubmed ID: 27934869 Three-Dimensionally Functionalized Reverse Phase Glycoprotein Array for Cancer Biomarker Discovery and Validation Journal of the American Chemical Society. 11, 2016 | Pubmed ID: 27933927 BNIP3 Protein Suppresses PINK1 Kinase Proteolytic Cleavage to Promote Mitophagy The Journal of Biological Chemistry. Oct, 2016 | Pubmed ID: 27528605 Multiplexed Imaging of Protein Phosphorylation on Membranes Based on Ti(IV) Functionalized Nanopolymers Chembiochem : a European Journal of Chemical Biology. 05, 2016 | Pubmed ID: 27037847 The E3 Ubiquitin Ligase CHIP Mediates Ubiquitination and Proteasomal Degradation of PRMT5 Biochimica Et Biophysica Acta. Feb, 2016 | Pubmed ID: 26658161 Identification of Direct Kinase Substrates Via Kinase Assay-Linked Phosphoproteomics Methods in Molecular Biology (Clifton, N.J.). 2016 | Pubmed ID: 26584932 MET18 Connects the Cytosolic Iron-Sulfur Cluster Assembly Pathway to Active DNA Demethylation in Arabidopsis PLoS Genetics. Oct, 2015 | Pubmed ID: 26492035 Time-Resolved Proteomic Visualization of Dendrimer Cellular Entry and Trafficking Journal of the American Chemical Society. Oct, 2015 | Pubmed ID: 26425924 In-Depth Analyses of B Cell Signaling Through Tandem Mass Spectrometry of Phosphopeptides Enriched by PolyMAC International Journal of Mass Spectrometry. Feb, 2015 | Pubmed ID: 25954137 Sensitive Measurement of Total Protein Phosphorylation Level in Complex Protein Samples The Analyst. May, 2015 | Pubmed ID: 25857711 Universal Non-antibody Detection of Protein Phosphorylation Using PIMAGO Current Protocols in Chemical Biology. Mar, 2015 | Pubmed ID: 25727060 The Methyl-CpG-binding Protein MBD7 Facilitates Active DNA Demethylation to Limit DNA Hyper-methylation and Transcriptional Gene Silencing Molecular Cell. Mar, 2015 | Pubmed ID: 25684209 The Sensor Histidine Kinase RgfC Affects Group B Streptococcal Virulence Factor Expression Independent of Its Response Regulator RgfA Infection and Immunity. Mar, 2015 | Pubmed ID: 25561709 Nitric Oxide Negatively Regulates Abscisic Acid Signaling in Guard Cells by S-nitrosylation of OST1 Proceedings of the National Academy of Sciences of the United States of America. Jan, 2015 | Pubmed ID: 25550508 Identification of ERK1 Direct Substrates Using Stable Isotope Labeled Kinase Assay-Linked Phosphoproteomics Molecular & Cellular Proteomics : MCP. Jul, 2014 | Pubmed ID: 25022875 Specific Visualization and Identification of Phosphoproteome in Gels Analytical Chemistry. Jul, 2014 | Pubmed ID: 24941108 Global Phosphoproteomics of Activated B Cells Using Complementary Metal Ion Functionalized Soluble Nanopolymers Analytical Chemistry. Jul, 2014 | Pubmed ID: 24905233 Quantitation of the Phosphoproteome Using the Library-assisted Extracted Ion Chromatogram (LAXIC) Strategy Methods in Molecular Biology (Clifton, N.J.). 2014 | Pubmed ID: 24792004 Tissue Phosphoproteomics with PolyMAC Identifies Potential Therapeutic Targets in a Transgenic Mouse Model of HER2 Positive Breast Cancer Electrophoresis. Dec, 2014 | Pubmed ID: 24723360 Current Technologies to Identify Protein Kinase Substrates in High Throughput Frontiers in Biology. Apr, 2013 | Pubmed ID: 25110472 Phosphatase of Regenerating Liver 3 (PRL3) Provokes a Tyrosine Phosphoproteome to Drive Prometastatic Signal Transduction Molecular & Cellular Proteomics : MCP. Dec, 2013 | Pubmed ID: 24030100 Intracellular Targets for a Phosphotyrosine Peptidomimetic Include the Mitotic Kinesin, MCAK Biochemical Pharmacology. Sep, 2013 | Pubmed ID: 23830822 Identification of Direct Tyrosine Kinase Substrates Based on Protein Kinase Assay-linked Phosphoproteomics Molecular & Cellular Proteomics : MCP. Oct, 2013 | Pubmed ID: 23793017 Quantitative Phosphoproteomics Identifies SnRK2 Protein Kinase Substrates and Reveals the Effectors of Abscisic Acid Action Proceedings of the National Academy of Sciences of the United States of America. Jun, 2013 | Pubmed ID: 23776212 Quantitative Measurement of Phosphoproteome Response to Osmotic Stress in Arabidopsis Based on Library-Assisted EXtracted Ion Chromatogram (LAXIC) Molecular & Cellular Proteomics : MCP. Aug, 2013 | Pubmed ID: 23660473 A Quantitative Proteomics-based Competition Binding Assay to Characterize PITAM-protein Interactions Analytical Chemistry. May, 2013 | Pubmed ID: 23611696 Syk Inhibits the Activity of Protein Kinase A by Phosphorylating Tyrosine 330 of the Catalytic Subunit The Journal of Biological Chemistry. Apr, 2013 | Pubmed ID: 23447535 2012 ASMS Fall Workshop: Mass Spectrometry-based Phosphorylation Analysis and Phosphoproteomics Journal of the American Society for Mass Spectrometry. Mar, 2013 | Pubmed ID: 23381688 Is Phosphoproteomics Ready for Clinical Research? Clinica Chimica Acta; International Journal of Clinical Chemistry. May, 2013 | Pubmed ID: 23159844 Identification of the Components of a Glycolytic Enzyme Metabolon on the Human Red Blood Cell Membrane The Journal of Biological Chemistry. Jan, 2013 | Pubmed ID: 23150667 Multiplexed Quantitation of Protein Expression and Phosphorylation Based on Functionalized Soluble Nanopolymers Journal of the American Chemical Society. Nov, 2012 | Pubmed ID: 23088311 Identification of Cytoskeletal Elements Enclosing the ATP Pools That Fuel Human Red Blood Cell Membrane Cation Pumps Proceedings of the National Academy of Sciences of the United States of America. Jul, 2012 | Pubmed ID: 22745158 Chemical Visualization of Phosphoproteomes on Membrane Molecular & Cellular Proteomics : MCP. Sep, 2012 | Pubmed ID: 22593177 Sensitive Kinase Assay Linked with Phosphoproteomics for Identifying Direct Kinase Substrates Proceedings of the National Academy of Sciences of the United States of America. Apr, 2012 | Pubmed ID: 22451900 Regulation of Parkin and PINK1 by Neddylation Human Molecular Genetics. Jun, 2012 | Pubmed ID: 22388932 Functionalized Soluble Nanopolymers for Phosphoproteome Analysis Methods in Molecular Biology (Clifton, N.J.). 2011 | Pubmed ID: 21948422 Direct Detection of Fatty Acid Ethyl Esters Using Low Temperature Plasma (LTP) Ambient Ionization Mass Spectrometry for Rapid Bacterial Differentiation The Analyst. Aug, 2011 | Pubmed ID: 21706093 Facile Determination of Double Bond Position in Unsaturated Fatty Acids and Esters by Low Temperature Plasma Ionization Mass Spectrometry Analytical Chemistry. Jun, 2011 | Pubmed ID: 21539336 Aptamer in Bioanalytical Applications Analytical Chemistry. Jun, 2011 | Pubmed ID: 21524128 Proteomic Studies of Syk-interacting Proteins Using a Novel Amine-specific Isotope Tag and GFP Nanotrap Journal of the American Society for Mass Spectrometry. Feb, 2011 | Pubmed ID: 21472591 Identification of Drug Targets in Vitro and in Living Cells by Soluble-nanopolymer-based Proteomics Angewandte Chemie (International Ed. in English). Apr, 2011 | Pubmed ID: 21455918 Phosphorylation Assay Based on Multifunctionalized Soluble Nanopolymer Analytical Chemistry. Apr, 2011 | Pubmed ID: 21395237 Purification and Characterization of a Metalloproteinase, Porthidin-1, from the Venom of Lansberg's Hog-nosed Pitvipers (Porthidium Lansbergii Hutmanni) Toxicon : Official Journal of the International Society on Toxinology. Mar, 2011 | Pubmed ID: 21255600 Regulation of Syk by Phosphorylation on Serine in the Linker Insert The Journal of Biological Chemistry. Dec, 2010 | Pubmed ID: 20956537 Cloning, Expression, and Hemostatic Activities of a Disintegrin, R-mojastin 1, from the Mohave Rattlesnake (Crotalus Scutulatus Scutulatus) Thrombosis Research. Sep, 2010 | Pubmed ID: 20598348 In-depth Analyses of Kinase-dependent Tyrosine Phosphoproteomes Based on Metal Ion-functionalized Soluble Nanopolymers Molecular & Cellular Proteomics : MCP. Oct, 2010 | Pubmed ID: 20562096 Identification of Serine/threonine Kinase Substrates in the Human Pathogen Group B Streptococcus Journal of Proteome Research. May, 2009 | Pubmed ID: 19309132 Quantitative Phospho-proteomics Based on Soluble Nanopolymers Methods in Molecular Biology (Clifton, N.J.). 2009 | Pubmed ID: 19241010 Playing Tag with Quantitative Proteomics Analytical and Bioanalytical Chemistry. Jan, 2009 | Pubmed ID: 18843486 Quantitative Analysis of Snake Venoms Using Soluble Polymer-based Isotope Labeling Molecular & Cellular Proteomics : MCP. Apr, 2008 | Pubmed ID: 18089550 Soluble Polymer-based Isotopic Labeling (SoPIL): a New Strategy to Discover Protein Biomarkers? Expert Review of Proteomics. Oct, 2007 | Pubmed ID: 17941815 Profiling Constitutive Proteolytic Events in Vivo The Biochemical Journal. Oct, 2007 | Pubmed ID: 17650073 Soluble Nanopolymer-based Phosphoproteomics for Studying Protein Phosphatase Methods (San Diego, Calif.). Jul, 2007 | Pubmed ID: 17532516 A Novel Quantitative Proteomics Reagent Based on Soluble Nanopolymers Chemical Communications (Cambridge, England). Mar, 2007 | Pubmed ID: 17356773 Rapid Ambient Mass Spectrometric Profiling of Intact, Untreated Bacteria Using Desorption Electrospray Ionization Chemical Communications (Cambridge, England). Jan, 2007 | Pubmed ID: 17279261 PTEN-deficient Intestinal Stem Cells Initiate Intestinal Polyposis Nature Genetics. Feb, 2007 | Pubmed ID: 17237784 A Novel Quantitative Proteomics Strategy to Study Phosphorylation-dependent Peptide-protein Interactions Journal of Proteome Research. Jan, 2007 | Pubmed ID: 17203957 Polar Acetalization and Transacetalization in the Gas Phase: the Eberlin Reaction Chemical Reviews. Jan, 2006 | Pubmed ID: 16402776 Quantitative Phosphoproteome Analysis Using a Dendrimer Conjugation Chemistry and Tandem Mass Spectrometry Nature Methods. Aug, 2005 | Pubmed ID: 16094384 Chiral Analysis by MS Analytical Chemistry. Jan, 2003 | Pubmed ID: 12530808 Ligand and Metal-ion Effects in Metal-ion Clusters Used for Chiral Analysis of Alpha-hydroxy Acids by the Kinetic Method Analytical and Bioanalytical Chemistry. Aug, 2002 | Pubmed ID: 12185574 Quotient Ratio Method for Quantitative Enantiomeric Determination by Mass Spectrometry Analytical Chemistry. Aug, 2002 | Pubmed ID: 12175167 Parallel Reactions for Enantiomeric Quantification of Peptides by Mass Spectrometry This Work Was Supported by the U.S. Department of Energy, Office of Energy Research. W.A.T. Acknowledges Fellowship Support from Triangle Pharmaceuticals. Angewandte Chemie (International Ed. in English). Feb, 2001 | Pubmed ID: 11241614 Replacement of C-O by P-O in Cyclic Acetals and Ketals Angewandte Chemie (International Ed. in English). Feb, 1999 | Pubmed ID: 29711658 Synthesis of B- and P-Heterocycles by Reaction of Cyclic Acetals and Ketals with Borinium and Phosphonium Ions The Journal of Organic Chemistry. Apr, 1999 | Pubmed ID: 11674423 Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis Chuan-Chih Hsu1,3, Chia-Feng Tsai2, W. Andy Tao3,4, Pengcheng Wang5 1Department of Plant Biology, Carnegie Institute for Science, 2Graduate School of Pharmaceutical Sciences, Kyoto University, 3Department of Biochemistry, Purdue University, 4Department of Chemistry, Purdue University, 5Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences JoVE 61489 Biochemistry
Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis Chuan-Chih Hsu1,3, Chia-Feng Tsai2, W. Andy Tao3,4, Pengcheng Wang5 1Department of Plant Biology, Carnegie Institute for Science, 2Graduate School of Pharmaceutical Sciences, Kyoto University, 3Department of Biochemistry, Purdue University, 4Department of Chemistry, Purdue University, 5Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences JoVE 61489 Biochemistry