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Epigenetic Inheritance and ChIP in Caenorhabditis elegans

RNAi Inheritance and ChIP in C. elegans (Video) | JoVE

RNAi Inheritance and ChIP in C. elegans (Video) | JoVE

01:27 min

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Bleaching and Plating Caenorhabditis elegans P0 Generation Embryos onto RNAi-NGM plates (Video) | JoVE

Bleaching and Plating Caenorhabditis elegans P0 Generation Embryos onto RNAi-NGM plates (Video) | JoVE

02:36 min

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Passaging and Scoring the Caenorhabditis elegans P0 Generation for Germline GFP Expression (Video) | JoVE

Passaging and Scoring the Caenorhabditis elegans P0 Generation for Germline GFP Expression (Video) | JoVE

02:02 min

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Collection of F1 Generation Caenorhabditis elegans for Chromatin Immunoprecipitation (ChIP) (Video) | JoVE

Collection of F1 Generation Caenorhabditis elegans for Chromatin Immunoprecipitation (ChIP) (Video) | JoVE

00:59 min

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Formaldehyde Crosslinking of the F1 Generation of Caenorhabditis elegans for ChIP (Video) | JoVE

Formaldehyde Crosslinking of the F1 Generation of Caenorhabditis elegans for ChIP (Video) | JoVE

01:18 min

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Sonication of Formaldehyde-Crosslinked Caenorhabditis elegans and Chromatin Immunoprecipitation (Video) | JoVE

Sonication of Formaldehyde-Crosslinked Caenorhabditis elegans and Chromatin Immunoprecipitation (Video) | JoVE

02:41 min

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Chengyin Li*¹, Phoebe A. W. Bhagoutie*¹, Victor Lao¹, Arneet L. Saltzman¹

¹Department of Cell and Systems Biology, University of Toronto

* These authors contributed equally

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פרוטוקול זה מתאר התאבכות RNA ובדיקת ChIP כדי לחקור את התורשה האפיגנטית של השתקה הנגרמת על ידי RNAi ושינויים כרומטינים הקשורים ב- C. elegans.

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