Assessing Serum Neutralization Against SARS-CoV-2 with Pseudotyped Viruses

November 21st, 2023

Studying Host-Virus Interactions with Pseudotyped Viruses (Video) | JoVE

01:03 min

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SARS-CoV-2 Pseudotype Virus Production and Serum Neutralization Assay (Video) | JoVE

04:50 min

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Tobia Fantoni*¹, Michele Bissoli*¹, Chiara Stefani¹, Mauro Voi¹, Alexandrina Dabija¹, Rebecca Casula¹, Domenico Luca Minafra¹, Julys da Fonseca Palmeira², Enrique Roberto Argañaraz², Martin Mayora-Neto³, Nigel J. Temperton³, Donato Zipeto¹, Alessandra Ruggiero¹

¹Department of Neuroscience, Biomedicine and Movement, University of Verona, ²Laboratory of Molecular Neurovirology, Faculty of Health Science, University of Brasília, ³Viral Pseudotype Unit, Medway School of Pharmacy, Universities of Kent and Greenwich at Medway

* These authors contributed equally

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Pseudotypade virus (PV) är replikationsdefekta virioner som används för att studera värd-virusinteraktioner under säkrare förhållanden än vid hantering av autentiska virus. Här presenteras ett detaljerat protokoll som visar hur SARS-CoV-2 PV kan användas för att testa den neutraliserande förmågan hos patienters serum efter COVID-19-vaccination.

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Assessing Serum Neutralization Against SARS-CoV-2 with Pseudotyped Viruses

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