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Using Induced Pluripotent Stem Cell Suspension for Kidney Organoid Production

September 1st, 2023
*1,2, *3, 4, 2, 1, 1, 1, 1, 5, 1, 1,2
1Department of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, 2Zhejiang University School of Medicine, 3Hubei Normal University, 4National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, 5Clinical Laboratory, The Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health
* These authors contributed equally

Summary
Automatic Translation

Detta protokoll presenterar en omfattande och effektiv metod för att producera njurorganoider från inducerade pluripotenta stamceller (iPSC) med hjälp av suspensionsodlingsförhållanden. Den primära tyngdpunkten i denna studie ligger i bestämningen av den initiala celltätheten och koncentrationen av WNT-agonisten, vilket gynnar forskare som är intresserade av forskning om njurorganoider.

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