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Generation of and Comparison Between Patient-Derived Gastric Organoids from Different Regions of the Stomach (Video) | JoVE
Isolating Single Cells from Upper Endoscopy Biopsies Derived from Gastric Patients for Organoid Generation (Video) | JoVE
Embedding Gastric Biopsy-Derived Single Cells in a Basement Membrane Matrix Dome for Organoid Generation (Video) | JoVE
Routine Passaging of Patient-Derived Gastric Organoids Through Fragmentation (Video) | JoVE
胃患者来源的类器官在研究中得到了越来越多的应用,但缺乏从具有标准化接种密度的单细胞消化物中生成人胃类器官的正式方案。该协议提出了一种从上消化道内窥镜检查期间获得的活检组织可靠地创建胃类器官的详细方法。
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