Materials
Name | Company | Catalog Number | Comments |
Iscove's Modified Dulbecco's Medium w/ NaHCO3 & 25mM Hepes | Life Technologies | 12440079 | |
Amphotericin B Solution | Sigma-Aldrich | A2942 | |
Penicillin/Streptomycin 100x Solution | Life Technologies | 10378-016 | |
Fetal Bovine Serum | Sigma-Aldrich | 12105 | |
Branson SLPe 40kHz Cell Disruptor with 3" (25mm) Cuphorn | Branson Ultrasonics | 101-063-726 | sonication device |
Brisk Heat SDC Benchtop Digital temperature Controler w/ 1000 ml Beaker Heater | Brisk Heat | SDCJF1A-GBH1000-1 | heater used for temperature control |
Beckman-Coulter Z2 Cell Sizer with AccuComp® Software | Beckman-Coulter | 6605700 | |
Bio-Rad TC20 Automated Cell Counter | Bio-Rad | 145-0102 | |
Gentamicin 50 mg/ml | Sigma-Aldrich | G1397 | |
Trypan Blue Solution | Sigma-Aldrich | T8154 | |
Falcon 50 ml & 25 ml Vented Culture Flasks | Fisher Scientific | 353082 | |
Lonza L-Glutamine 200 mM 0.85% NaCl | Lonza | 17-605C | |
Seal-Rite 1.5 ml Microcentrifuge Tubes | USA Scientific | 1615-5510 | |
Beckman-Coulter Accuvette ST 25 ml Vials and caps | Beckman-Coulter | A35473 | |
AccuJet Pro Auto Pipet | BrandTech Scientific | 26330 | |
USA Scientific 10 ml Disposable Serological Pipets | USA Scientific | 1071-0810 | |
Tip One 100 μl and 1,000 μl Filter Tips | USA Scientific | 1120-1840, 1126-7810 | |
100 μl Micropipette | Wheaton | 851164 | |
1,000 μl Micropipette | Wheaton | 851168 | |
BioRad Dual Chamber Counting Slides | Bio-Rad | 145-0015 | |
Forma Scientific Dual chamber water jacketed Incubator | Forma Scientific | 3131 | |
Tektronix DPO 2002B Digital Phosphor Oscilloscope | Tektronix | DPO2002B | used to measure the ultrasonic waveform |
PPB MegaSonics Model PB-500 Ultrasonic Energy Meter | PPB Megasonics | PB-500 | used to assess the sound intensity in W/cm2 |
Teledyne RESON TC4013-1 Hydrophone | Teledyne | TC4013-1 | connects to the oscilloscope |
Wheaton 250 ml Flasks | Sigma-Aldrich | Z364827 | |
20 ml Glass Scintillation Vials | Sigma-Aldrich | Z190527 | |
Beckman-Coulter Isotonic Saline Solution | Beckman-Coulter | N/A | diluent for Z2 counter |
Chloroform 99% | Sigma-Aldrich | C2432 | |
Ethanol 200 Proof Anhydrous | Sigma-Aldrich | 459836 | |
Mineral Oil | N/A | ||
XTT Cell Proliferation Assay Kit | ATCC | 30-1011K | |
96-Well Microplate Reader | Cole-Palmer | EW-13055-54 | |
U937 Human Monocytic Leukemia Cells | ATCC | CRL1593.2 | |
THP1 Human Monocytic Leukemia Cells | ATCC | TIB-202 |
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