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Journal / Biology / In vivo elektroporering av Morpholinos i Adult…
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Biology

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In vivo elektroporering av Morpholinos i Adult Zebrafish Retina
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In vivo elektroporering av Morpholinos i Adult Zebrafish Retina

Article DOI: 10.3791/3603-v • 06:55 min • December 27th, 2011
December 27th, 2011 •
1, 2,3, 2,3
1Departments of Anatomy and Cell Biology and Ophthalmology, Wayne State University School of Medicine, 2Department of Biological Sciences, University of Notre Dame, 3Center for Zebrafish Research, University of Notre Dame

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En metod för att villkorligt ÖVERVÄLDIGANDE ett målprotein uttryck i den vuxna zebrafisk näthinnan beskrivs, som innebär intravitreally injicera antisense morpholinos och electroporating dem i näthinnan. Den resulterande protein är knockad i flera dagar, vilket gör att testa proteinets roll i regenererande eller intakt näthinnan.

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Utvecklingsbiologi elektroporation morpholino zebrafisk näthinnan förnyelse
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