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JoVE Journal
Immunology and Infection

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High-throughput Screening for Bredspektret Chemical hæmmere af RNA virus
 
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High-throughput Screening for Bredspektret Chemical hæmmere af RNA virus

Article DOI: 10.3791/51222-v 11:34 min May 5th, 2014
May 5th, 2014

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Summary

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Click here for the English version.

In vitro assays til at måle virus replikation er blevet kraftigt forbedret gennem udvikling af rekombinante RNA-virus, der udtrykker luciferase eller andre enzymer, der kan bioluminescens. Her har vi detalje en high-throughput screening rørledning, der kombinerer disse rekombinante stammer af mæslinger og Chikungunya virus at isolere bredspektrede antivirale fra kemiske biblioteker.

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Immunologi virusinfektioner high-throughput screening assays bredspektrede antivirale chikungunya mæslingevirus luciferase reporter kemiske biblioteker
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