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JoVE Journal Neuroscience
Completa lesioni del midollo spinale e del cervello dissezione Protocollo per Wholemount successiva
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Completa lesioni del midollo spinale e del cervello dissezione Protocollo per Wholemount successiva

Article DOI: 10.3791/51494-v • 08:11 min • October 14th, 2014
October 14th, 2014 •
1, 2, 2,3, 2
1Centre for Neuroregeneration, School of Biomedical Sciences, University of Edinburgh, 2Shriners Hospitals Pediatric Research Center (Center for Neural Repair and Rehabilitation), Temple University School of Medicine, 3Department of Neurology, Temple University School of Medicine

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Lamprede recuperare locomozione dopo una lesione completa del midollo spinale. Tuttavia, alcuni neuroni spinali-proiezione sono buoni recuperatori e altri non lo sono. Questo documento illustra le tecniche per la lampreda di mare abitazioni larve (e adulti recentemente trasformati), producendo completi transezioni midollo spinale e la preparazione di cervelli wholemount e midolli spinali per l'ibridazione in situ.

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Neuroscienze lesioni del midollo spinale le molecole di orientamento assonale neurofilamenti la rigenerazione
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