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Journal / Developmental Biology / Utilizando Análise de confocal
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Developmental Biology

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JoVE Journal Developmental Biology
Utilizando Análise de confocal
Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions
Journal (Developmental Biology)
Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions
Fast and Efficient Expression of Multiple Proteins in Avian Embryos Using mRNA Electroporation
Journal (Developmental Biology)
Fast and Efficient Expression of Multiple Proteins in Avian Embryos Using mRNA Electroporation
 
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Utilizando Análise de confocal

Article DOI: 10.3791/53162-v • 08:10 min • December 14th, 2015
December 14th, 2015 •
1, 2, 3, 4, 5, 5
1Department of Biomedical Science, The Bateson Centre, University of Sheffield, 2Institute of Genetic Medicine, Newcastle University, 3Department of Cardiovascular Science, The Bateson Centre, University of Sheffield, 4School of Biochemistry, University of Bristol, 5Biology Department, University of York

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O manuscrito aqui fornece um simples conjunto de métodos que permitam analisar a secreção e difusão de ligantes marcados fluorescentes em Xenopus. Isto proporciona um contexto para testar a capacidade de outras proteínas para modificar a distribuição de ligando e permitindo experiências que podem dar uma visão sobre os mecanismos que regulam a gradientes de morfogénio.

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Biologia do Desenvolvimento edição 106 Hedgehog sem asas GFP RFP biologia do desenvolvimento padronização ligantes secretados sinalização celular
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