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Chromatin Immunutfelling analyse for identifikasjon av Arabidopsis Protein-DNA interaksjoner
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Chromatin Immunutfelling analyse for identifikasjon av Arabidopsis Protein-DNA interaksjoner

Article DOI: 10.3791/53422-v • 12:36 min • January 14th, 2016
January 14th, 2016 •
1, 1, 1, 1
1Centro de Biotecnología y Genómica de Plantas (CBGP), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)-Universidad Politécnica de Madrid

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Kromatin immunoutfellingen er en kraftfull teknikk for identifisering av DNA-bindende områder av Arabidopsis proteiner in vivo. Denne fremgangsmåten omfatter kromatin tverrbinding og fragmentering, immunopresipitering med selektive antistoffer mot proteinet av interesse, og qPCR-analyse av bundet DNA. Vi beskriver en enkel ChIP assay optimalisert for Arabidopsis planter.

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Plant Biology Developmental Biology, Chromatin immunopresipitering (chip) transkripsjonsregulering blomstringstid
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