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Journal / Immunology and Infection / Applicando Fluorescence Resonance Energy Transfer …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Applicando Fluorescence Resonance Energy Transfer (FRET) per esaminare Effector Translocation efficienza da
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Journal (Immunology and Infection)
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Journal (Immunology and Infection)
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Applicando Fluorescence Resonance Energy Transfer (FRET) per esaminare Effector Translocation efficienza da

Article DOI: 10.3791/54210-v • 10:29 min • July 6th, 2016
July 6th, 2016 •
1, 1, 1
1Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne

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Indagare le interazioni tra batteri patogeni ei loro ospiti è un'area importante della ricerca biologica. Qui, descriviamo le tecniche necessarie per misurare effettrici traslocazione da Coxiella burnetii durante siRNA silenziamento genico mediante substrato Blam.

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L'infezione interazioni ospite-patogeno sistemi di secrezione batterici test traslocazione β-lattamasi Blam substrato fluorescenza microbiologia batteri patogeni intracellulari, Silenziamento genico siRNA biologia molecolare
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