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JoVE Journal Developmental Biology
Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption
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Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption

Article DOI: 10.3791/54228-v • 12:02 min • July 30th, 2016
July 30th, 2016 •
*1, *1, *1, 1, 1, 1, 1, 1, 1, 1
1INRS-Institut Armand-Frappier
* These authors contributed equally

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Summary
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This manuscript presents a unique in vitro model of immunopurified human villous cytotrophoblast cells cultured under hypoxia/reoxygenation. This model is suitable to study the protective effects of promising treatments, such as melatonin, on pregnancy complications associated with increased oxidative stress and altered placental function.

Tags

Human Primary Trophoblast Cell Culture Melatonin Hypoxia/reoxygenation Protective Effects Pregnancy Complications Placenta Oxidative Stress Villous Cytotrophoblast Isolation Density Centrifugation Median Gradient Third Trimester Trophoblast First Trimester Placenta Visual Demonstration Graduate Students Umbilical Cord Histological Analysis
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