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Journal / Biology / 监测ER / SR钙释放与目标Ca
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监测ER / SR钙释放与目标Ca
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监测ER / SR钙释放与目标Ca

Article DOI: 10.3791/55822-v • 12:30 min • May 19th, 2017
May 19th, 2017 •
1, 1, 1, 1
1Department of Chemistry, Center of Diagnostics and Therapeutics (CDT), Georgia State University

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我们提出了使用我们的靶向基因编码钙指示剂（GECI）CatchER +的方案 ，用于使用实时荧光显微镜监测HEK293和骨骼肌C2C12细胞的内质网/肌浆网（ER / SR）中的快速钙瞬变。还讨论了原位测量和校准的协议。

Tags

生物化学，第123期，钙成像，肌质内质网，遗传编码钙指示剂，钙信号，CatchER，荧光显微镜
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