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Journal / Biochemistry / 利用综合沉淀富集系统识别目标蛋白的内源性后向转化剖面…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
利用综合沉淀富集系统识别目标蛋白的内源性后向转化剖面
Arteriovenous Blood Sampling Technique in Mice Using Sulzer's Vein and Left Ventricle for Metabolomic Analysis (Video) | JoVE
Journal (Biochemistry)
Arteriovenous Blood Sampling Technique in Mice Using Sulzer's Vein and Left Ventricle for Metabolomic Analysis (Video) | JoVE
Quantifying Bacterial Surface Swarming Motility on Inducer Gradient Plates
Journal (Biochemistry)
Quantifying Bacterial Surface Swarming Motility on Inducer Gradient Plates
 
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利用综合沉淀富集系统识别目标蛋白的内源性后向转化剖面

Article DOI: 10.3791/56912-v • 08:12 min • January 8th, 2018
January 8th, 2018 •
1, 1, 1
1R&D Department, Cytoskeleton Inc.

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研究多, 内源性修饰修改的目标蛋白可能是极具挑战性的。这里描述的技术利用一个优化的裂解缓冲和过滤系统开发了特定的 PTM 靶向, 亲和矩阵, 以检测乙酰化, 泛, sumo 2/3 和酪氨酸磷酸酯的修改为目标蛋白质使用单一的, 流线型的系统。

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生物化学 问题 131 后平移修饰 PTM 检测 乙酰化 泛 磷酸化 sumo 基因组 DNA 去除 变性裂解缓冲 沉淀 PTM IP 协议
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