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Journal / Genetics / RNA sentezi 5-Bromouridine etiketleme ve…
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Genetics

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JoVE Journal Genetics
RNA sentezi 5-Bromouridine etiketleme ve Immunoprecipitation kullanarak incelenmesi
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RNA sentezi 5-Bromouridine etiketleme ve Immunoprecipitation kullanarak incelenmesi

Article DOI: 10.3791/57056-v • 09:59 min • May 3rd, 2018
May 3rd, 2018 •
1, 1, 2, 2, 1
1DANDRITE - Danish Research Institute of Translational Neuroscience, Dept. of Biomedicine, Aarhus University, 2Dept. of Molecular Biology and Genetics, Aarhus University

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Bu yöntem RNA sentezi ölçmek için kullanılabilir. 5-Bromouridine hücrelere eklendi ve sentezlenmiş RNA dahil. RNA sentezi 5 Bromouridine hedeflenmiş immunoprecipitation etiketli RNA ve analiz ters transkripsiyon ve nicel Polimeraz zincir reaksiyonu ardından hemen sonra etiketleme, RNA ayıklama ölçülür.

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Genetik sorunu 135 RNA sentezi 5-Bromouridine etiketleme immunoprecipitation fenol kloroform ayıklama ters transkripsiyon nicel Polimeraz zincir reaksiyonu
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