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Journal / Immunology and Infection / In Vitro Hjørnetann nøytrofile ekstracellulære…
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Immunology and Infection

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In Vitro Hjørnetann nøytrofile ekstracellulære felle formasjon
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In Vitro Hjørnetann nøytrofile ekstracellulære felle formasjon: Dynamisk og kvantitativ analyse av fluorescens mikroskopi

Article DOI: 10.3791/58083-v • 09:45 min • August 24th, 2018
August 24th, 2018 •
1, 2
1Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, 2Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis

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Vi beskriver metoder for å isolere hjørnetann nøytrofile fra hele blod og visualisere netto formasjon i live nøytrofile bruker fluorescens mikroskopi. Også beskrevet er protokoller å kvantifisere netto dannelse og citrullinated histone H3 (citH3) uttrykk med immunofluorescence mikroskopi.

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Immunologi og infeksjon problemet 138 Immunofluorescence mikroskopi sepsis granulocytt isolasjon histone citrullination peptidylarginine deiminase
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