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Journal / Biology / Analisi di assorbimento di colesterolo LDL…
JoVE Journal
Biology

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JoVE Journal Biology
Analisi di assorbimento di colesterolo LDL utilizzando Live Cell Imaging analisi con cella di monitoraggio dello stato
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Analisi di assorbimento di colesterolo LDL utilizzando Live Cell Imaging analisi con cella di monitoraggio dello stato

Article DOI: 10.3791/58564-v • 08:45 min • November 17th, 2018
November 17th, 2018 •
*1,2, *1,3, 4, 4,5, 6, 1,2,7,8
1Interdisciplinary Stem Cell Institute, University of Miami Leonard M. Miller School of Medicine, 2Department of Medicine, Division of Cardiology, University of Miami Leonard M. Miller School of Medicine, 3Department of Molecular and Cellular Pharmacology, University of Miami Leonard M. Miller School of Medicine, 4Dr. John T. Macdonald Foundation Department of Human Genetics, University of Miami Leonard M. Miller School of Medicine, 5John P. Hussman Institute for Human Genomics, University of Miami Leonard M. Miller School of Medicine, 6Department of Medicine, Division of Endocrinology, Metabolism and Endocrinology and the Diabetes Research Institute, University of Miami Leonard M. Miller School of Medicine, 7Vascular Biology Institute, University of Miami Leonard M. Miller School of Medicine, 8Peggy and Harold Katz Family Drug Discovery Center, University of Miami Leonard M. Miller School of Medicine
* These authors contributed equally

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Summary
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Questo protocollo fornisce un approccio efficiente per misurare l'assorbimento del colesterolo di LDL con tariffe afflusso in tempo reale utilizzando una cellula viva imaging system in vari tipi cellulari. Questa tecnica fornisce una piattaforma per schermare l'attività farmacologica dei composti che interessano l'afflusso di LDL durante il monitoraggio per la morfologia delle cellule e quindi potenziale citotossicità.

Tags

Biologia problema 141 lipoproteina di densità bassa LDLR afflusso di LDL l'assorbimento di LDL Dynasore simvastatina ricombinante PCSK9 dinamina lipidi colesterolo aterosclerosi.
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