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Journal / Neuroscience / PiggyBac ベクターを用いたヒト誘導多能性幹細胞 (iPSCs)…
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Neuroscience

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JoVE Journal Neuroscience
PiggyBac ベクターを用いたヒト誘導多能性幹細胞 (iPSCs) の機能的脊髄および頭蓋運動ニューロンへの変換
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PiggyBac ベクターを用いたヒト誘導多能性幹細胞 (iPSCs) の機能的脊髄および頭蓋運動ニューロンへの変換

Article DOI: 10.3791/59321-v • 07:33 min • May 1st, 2019
May 1st, 2019 •
1, 2, 2, 2, 3, 2,4, 2,4, 1,2
1Department of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, Italy, 2Center for Life Nano Science, Istituto Italiano di Tecnologia, Italy, 3Laboratory of Stem Cell biology and Molecular Embryology, The Rockefeller University, USA, 4Department of Physiology and Pharmacology, Sapienza University of Rome, Italy

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このプロトコルは、誘導性 piggyBac ベクターからの転写因子を異所的に発現することによって、脊髄または頭蓋の同一性を有する運動ニューロンへの誘発多能性幹細胞の迅速かつ効率的な変換を可能にする。

Tags

神経科学、問題147、誘導多能性幹細胞、運動ニューロン、piggyBac、分化、転写因子、Phox2a、Isl1、Ngn2、Lhx3
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