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JoVE Journal Bioengineering
Etichette e acquisizioni Dextran nelle cellule dei capelli Murine Cochlear dal vivo e funzionali
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Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells
Journal (Bioengineering)
Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells
 
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Etichette e acquisizioni Dextran nelle cellule dei capelli Murine Cochlear dal vivo e funzionali

Article DOI: 10.3791/60769-v • 05:55 min • February 8th, 2020
February 8th, 2020 •
1, 1
1Molecular Physiology and Biophysics Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health

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Qui, presentiamo un metodo per visualizzare l'assorbimento di 3 kDa Texas Red-etichetta tono in cellule ciliate uditive con canali di meccanotrazione funzionali. Inoltre, dextrans di 3-10 kDa può essere utilizzato per studiare l'endocitosi nei capelli e le cellule di supporto dell'organo di Corti.

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Bioingegneria Problema 156 Dextran cellule ciliate dell'orecchio interno canale mechanotransduction assorbimento del colorante endocitosi vescicoli intracellulari microscopia confocale
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