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Visualisatie van replisoomontmoetingen met een antigeen gelabelde blokkerende laesie

Article DOI: 10.3791/61689-v • 08:24 min • July 27th, 2021
July 27th, 2021 •
*1, *2, 1, 3, 1, 4, 5, 6, 1, 1
1Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, 2Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, 3Department of Molecular Biology and Genetics, Cornell University, 4Department of Cellular and Molecular Medicine, University of Copenhagen, 5Horizon Discovery, 6Boston University School of Medicine
* These authors contributed equally

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Hoewel replicatievorkbotsingen met DNA-adducten dubbele strengsbreuken kunnen veroorzaken, is er minder bekend over de interactie tussen replisomen en blokkerende laesies. We hebben de proximity ligation assay gebruikt om deze ontmoetingen te visualiseren en de gevolgen voor de replisoomsamenstelling te karakteriseren.

Tags

Biologie replisome blocking laesion PLA proximity ligation assay DNA interstrand crosslinks antigen-tagged crosslinks
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